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mouse medchemexpress hy p73388 m csf protein  (MedChemExpress)


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    Structured Review

    MedChemExpress mouse medchemexpress hy p73388 m csf protein
    Mouse Medchemexpress Hy P73388 M Csf Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+m+csf+protein/RANKL%2FTNFSF11%2C+Mouse/pm41903530-172-7-8
    Average 94 stars, based on 3 article reviews
    mouse medchemexpress hy p73388 m csf protein - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Derivative Assay:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Cell Culture:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Control:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Incubation:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Two Tailed Test:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Expressing:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Activation Assay:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Fluorescence:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Marker:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Gene Expression:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    Migration:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.

    In Vivo:

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation
    Article Snippet: Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.Deionized (DI) water (18.2 MΩ· cm) was produced using a Milli-Q system (Millipore, Bedford, MA, USA) and used for all experiments.. Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.. The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.The following antibodies were obtained from BioLegend: APC anti-mouse F4/80, PE anti-mouse/human CD11b, Alexa Fluor® 647 anti-mouse CD74 (CLIP), APC anti-human CD163, Pacific BlueTM anti-mouse/human CD11b, Brilliant Violet 510TM anti-mouse CD45, FITC anti-mouse Ly-6C, and APC anti-mouse Ly-6G antibodies.

    Article Title: CEBPB-high dormant tumor cells drive immune evasion via S100A8 orchestrated tumor-associated macrophages reprogramming
    Article Snippet: The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).The BM cells were filtered through a 100 μm cell strainer, and red blood cells were removed using an RBC lysis buffer (eBioscience, #00-4300).. BM cells were then differentiated in complete DMEM medium supplemented with 20 ng/mL recombined mouse M-CSF protein (MCE, #HY-P70263A) for 7 days.. The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.The medium was changed every 3 days with fresh complete DMEM medium containing M-CSF.



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    a. Schematic illustration of strong confinement applied to freshly isolated mouse bone marrow–derived monocytes (mMonocytes) and human umbilical cord blood–derived monocytes (hMonocytes). b,c. Representative images (b) and cell circularity index (c) of mMonocytes, hMonocytes, <t>and</t> <t>M-CSF</t> – treated mMonocytes (20 ng/mL) cultured under untreated control (no confinement) or 3 μm strong confinement for long-term incubation. Scale bar, 10 μm. d. Representative images showing strong confinement–activated phagocytosis of cellular debris in mMonocytes. Scale bar, 20 μm. e. Representative images (left) and debris clearance efficiency (right) of mMonocytes under untreated control and strong confinement during ∼48 h live tracking. Data are shown as mean ± s.e.m. ( N > 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; *** P < 0.001. Scale bar, 50 μm. f. GO enrichment analysis of differentially expressed genes in strong confinement versus untreated control mMonocytes. g. Heat map showing the expression levels of macrophage activation – associated genes in mMonocytes under strong confinement versus untreated control conditions. h,i. GSEA of INFLAMMATORY_RESPONSE (h) and TNFA_SIGNALING_VIA_NFKB (i) gene sets in strong confinement versus untreated control mMonocytes. j. Representative images of macrophage markers F4/80 and CD163 in mMonocytes, hMonocytes, and M-CSF–treated mMonocytes under control and strong confinement conditions. Scale bar, 10 μm.
    Mouse M Csf Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a. Schematic illustration of strong confinement applied to freshly isolated mouse bone marrow–derived monocytes (mMonocytes) and human umbilical cord blood–derived monocytes (hMonocytes). b,c. Representative images (b) and cell circularity index (c) of mMonocytes, hMonocytes, and M-CSF – treated mMonocytes (20 ng/mL) cultured under untreated control (no confinement) or 3 μm strong confinement for long-term incubation. Scale bar, 10 μm. d. Representative images showing strong confinement–activated phagocytosis of cellular debris in mMonocytes. Scale bar, 20 μm. e. Representative images (left) and debris clearance efficiency (right) of mMonocytes under untreated control and strong confinement during ∼48 h live tracking. Data are shown as mean ± s.e.m. ( N > 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; *** P < 0.001. Scale bar, 50 μm. f. GO enrichment analysis of differentially expressed genes in strong confinement versus untreated control mMonocytes. g. Heat map showing the expression levels of macrophage activation – associated genes in mMonocytes under strong confinement versus untreated control conditions. h,i. GSEA of INFLAMMATORY_RESPONSE (h) and TNFA_SIGNALING_VIA_NFKB (i) gene sets in strong confinement versus untreated control mMonocytes. j. Representative images of macrophage markers F4/80 and CD163 in mMonocytes, hMonocytes, and M-CSF–treated mMonocytes under control and strong confinement conditions. Scale bar, 10 μm.

    Journal: bioRxiv

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation

    doi: 10.64898/2026.03.31.715742

    Figure Lengend Snippet: a. Schematic illustration of strong confinement applied to freshly isolated mouse bone marrow–derived monocytes (mMonocytes) and human umbilical cord blood–derived monocytes (hMonocytes). b,c. Representative images (b) and cell circularity index (c) of mMonocytes, hMonocytes, and M-CSF – treated mMonocytes (20 ng/mL) cultured under untreated control (no confinement) or 3 μm strong confinement for long-term incubation. Scale bar, 10 μm. d. Representative images showing strong confinement–activated phagocytosis of cellular debris in mMonocytes. Scale bar, 20 μm. e. Representative images (left) and debris clearance efficiency (right) of mMonocytes under untreated control and strong confinement during ∼48 h live tracking. Data are shown as mean ± s.e.m. ( N > 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; *** P < 0.001. Scale bar, 50 μm. f. GO enrichment analysis of differentially expressed genes in strong confinement versus untreated control mMonocytes. g. Heat map showing the expression levels of macrophage activation – associated genes in mMonocytes under strong confinement versus untreated control conditions. h,i. GSEA of INFLAMMATORY_RESPONSE (h) and TNFA_SIGNALING_VIA_NFKB (i) gene sets in strong confinement versus untreated control mMonocytes. j. Representative images of macrophage markers F4/80 and CD163 in mMonocytes, hMonocytes, and M-CSF–treated mMonocytes under control and strong confinement conditions. Scale bar, 10 μm.

    Article Snippet: Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.

    Techniques: Isolation, Derivative Assay, Cell Culture, Control, Incubation, Two Tailed Test, Expressing, Activation Assay

    a. Representative images of nuclear morphology in hMonocytes under control and strong confinement conditions. b. Representative images of maximum nuclear projected area in mMonocytes and hMonocytes (left), and quantitative analysis (right) in RAW264.7 cells, mMonocytes, and hMonocytes under control and strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 44 cells for RAW264.7-Ctrl, n = 61 cells for RAW264.7-Strong Conf.; n = 54 cells for mMonocytes-Ctrl, n = 68 cells for mMonocytes- Strong Conf.; n = 40 cells for hMonocytes-Ctrl, and n = 53 cells for hMonocytes- Strong Conf.). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001. Scale bar, 10 μm. c. GO enrichment analysis of differentially expressed genes associated with chromatin states in strong confinement versus control mMonocytes. d,e. Representative images (d) and relative fluorescence intensity analysis (e) of H3K27me3 in RAW264.7 cells, mMonocytes, M-CSF–treated mMonocytes, and M-CSF–treated hMonocytes under control and strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; RAW264.7: n = 44 cells for Ctrl and n = 61 cells for Strong Conf.; mMonocytes: n = 54 cells for Ctrl and n = 68 cells for Strong Conf.; mMonocytes + M-CSF: n = 56 cells for Ctrl and n = 66 cells for Strong Conf.; hMonocytes + M-CSF: n = 40 cells for Ctrl and n = 53 cells for Strong Conf.). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ***P < 0.001, ****P < 0.0001. Scale bar, 10 μm. f,g. Representative images (f) and correlation analysis (g) of H3K27me3 intensity and the macrophage marker F4/80 in mMonocytes after long-term strong confinement. Scale bar, 20 μm. h. Relative mRNA levels of Il6, Cxcl1, Tnfaip3, Tnf, Nos2, Il1b, and Csf1r in RAW264.7 cells and mMonocytes under control and strong confinement, showing activation of macrophage- and inflammation-associated genes. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. i,j. Heat map (i) and relative mRNA levels (j) showing expression of Ezh2, Suz12, Eed, Rbbp4, Rbbp7, Kdm6a, and Kdm6b in mMonocytes under control and strong confinement, highlighting confinement-induced alterations in Polycomb complex components and demethylases. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. k. Relative mRNA expression of Kdm6b in RAW264.7 cells and hMonocytes under control and strong confinement, confirming consistent upregulation across species and cell types. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001.

    Journal: bioRxiv

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation

    doi: 10.64898/2026.03.31.715742

    Figure Lengend Snippet: a. Representative images of nuclear morphology in hMonocytes under control and strong confinement conditions. b. Representative images of maximum nuclear projected area in mMonocytes and hMonocytes (left), and quantitative analysis (right) in RAW264.7 cells, mMonocytes, and hMonocytes under control and strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 44 cells for RAW264.7-Ctrl, n = 61 cells for RAW264.7-Strong Conf.; n = 54 cells for mMonocytes-Ctrl, n = 68 cells for mMonocytes- Strong Conf.; n = 40 cells for hMonocytes-Ctrl, and n = 53 cells for hMonocytes- Strong Conf.). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001. Scale bar, 10 μm. c. GO enrichment analysis of differentially expressed genes associated with chromatin states in strong confinement versus control mMonocytes. d,e. Representative images (d) and relative fluorescence intensity analysis (e) of H3K27me3 in RAW264.7 cells, mMonocytes, M-CSF–treated mMonocytes, and M-CSF–treated hMonocytes under control and strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; RAW264.7: n = 44 cells for Ctrl and n = 61 cells for Strong Conf.; mMonocytes: n = 54 cells for Ctrl and n = 68 cells for Strong Conf.; mMonocytes + M-CSF: n = 56 cells for Ctrl and n = 66 cells for Strong Conf.; hMonocytes + M-CSF: n = 40 cells for Ctrl and n = 53 cells for Strong Conf.). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ***P < 0.001, ****P < 0.0001. Scale bar, 10 μm. f,g. Representative images (f) and correlation analysis (g) of H3K27me3 intensity and the macrophage marker F4/80 in mMonocytes after long-term strong confinement. Scale bar, 20 μm. h. Relative mRNA levels of Il6, Cxcl1, Tnfaip3, Tnf, Nos2, Il1b, and Csf1r in RAW264.7 cells and mMonocytes under control and strong confinement, showing activation of macrophage- and inflammation-associated genes. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. i,j. Heat map (i) and relative mRNA levels (j) showing expression of Ezh2, Suz12, Eed, Rbbp4, Rbbp7, Kdm6a, and Kdm6b in mMonocytes under control and strong confinement, highlighting confinement-induced alterations in Polycomb complex components and demethylases. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. k. Relative mRNA expression of Kdm6b in RAW264.7 cells and hMonocytes under control and strong confinement, confirming consistent upregulation across species and cell types. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001.

    Article Snippet: Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.

    Techniques: Control, Two Tailed Test, Fluorescence, Marker, Activation Assay, Expressing

    a. Heat map showing gene expression profiles of mMonocytes pretreated with 20 ng/mL M-CSF for 48 h, cultured under strong confinement and treated with DMSO or 10 μM GSKJ4. b,c. GO enrichment analysis of genes from cluster 1 (b) and cluster 2 (c) identified in (a). d. Heat map showing the expression levels of macrophage activation – associated genes in mMonocytes pretreated with 20 ng/mL M-CSF for 48 h, cultured under strong confinement and treated with DMSO or 10 μM GSKJ4. e,f. Representative images (e) and quantitative analysis (f) of cell morphology in mMonocytes under DMSO or GSKJ4-treated strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments) Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar, 100 μm. g,h. qPCR analysis of Itgam (g) and Adgre1 (h) expression in control, strong confinement, and GSKJ4-treated strong confinement groups. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. i,j. Representative fluorescent images (i) showing F4/80 (red) and H3K27me3 (green), and mean migration speed analysis (j) of mMonocytes pretreated with 20 ng/mL M-CSF for 48 h under DMSO or GSKJ4-treated strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 248 cells for strong confinement and n = 241 cells for strong confinement + GSKJ4). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ***P < 0.001. Scale bar, 20 μm. k. Schematic illustration of the in vivo experimental workflow used to assess the effect of GSKJ4 on the maturation of liver capsular macrophages (top), together with representative two-photon intravital fluorescence images (bottom) showing Cx3cr1 (red–hot) and collagen fibers visualized by second harmonic generation (SHG) signals (cyan). Scale bar, 50 μm. l,m. Quantification of cell circularity (l) and cell area (m) of Cx3cr1 ⁺ liver capsular macrophages (LCMs) in vivo under vehicle control or GSKJ4 treatment. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 106 cells for vehicle control and n = 110 cells for GSKJ4 treatment). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001. n. Schematic model illustrating confinement-driven monocyte-to-macrophage differentiation mediated by Kdm6b-dependent epigenetic remodeling.

    Journal: bioRxiv

    Article Title: Mechanical confinement drives monocyte-to-macrophage differentiation

    doi: 10.64898/2026.03.31.715742

    Figure Lengend Snippet: a. Heat map showing gene expression profiles of mMonocytes pretreated with 20 ng/mL M-CSF for 48 h, cultured under strong confinement and treated with DMSO or 10 μM GSKJ4. b,c. GO enrichment analysis of genes from cluster 1 (b) and cluster 2 (c) identified in (a). d. Heat map showing the expression levels of macrophage activation – associated genes in mMonocytes pretreated with 20 ng/mL M-CSF for 48 h, cultured under strong confinement and treated with DMSO or 10 μM GSKJ4. e,f. Representative images (e) and quantitative analysis (f) of cell morphology in mMonocytes under DMSO or GSKJ4-treated strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments) Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar, 100 μm. g,h. qPCR analysis of Itgam (g) and Adgre1 (h) expression in control, strong confinement, and GSKJ4-treated strong confinement groups. Data are shown as mean ± s.e.m. ( N = 3 independent experiments). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001. i,j. Representative fluorescent images (i) showing F4/80 (red) and H3K27me3 (green), and mean migration speed analysis (j) of mMonocytes pretreated with 20 ng/mL M-CSF for 48 h under DMSO or GSKJ4-treated strong confinement conditions. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 248 cells for strong confinement and n = 241 cells for strong confinement + GSKJ4). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ***P < 0.001. Scale bar, 20 μm. k. Schematic illustration of the in vivo experimental workflow used to assess the effect of GSKJ4 on the maturation of liver capsular macrophages (top), together with representative two-photon intravital fluorescence images (bottom) showing Cx3cr1 (red–hot) and collagen fibers visualized by second harmonic generation (SHG) signals (cyan). Scale bar, 50 μm. l,m. Quantification of cell circularity (l) and cell area (m) of Cx3cr1 ⁺ liver capsular macrophages (LCMs) in vivo under vehicle control or GSKJ4 treatment. Data are shown as mean ± s.e.m. ( N = 3 independent experiments; n = 106 cells for vehicle control and n = 110 cells for GSKJ4 treatment). Statistical significance was determined by a two-tailed unpaired Student’s t -test; ****P < 0.0001. n. Schematic model illustrating confinement-driven monocyte-to-macrophage differentiation mediated by Kdm6b-dependent epigenetic remodeling.

    Article Snippet: Fibronectin, GSK-J4 hydrochloride, phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide (LPS; Escherichia coli O111:B4), and mouse M-CSF protein were purchased from MedChemExpress.

    Techniques: Gene Expression, Cell Culture, Expressing, Activation Assay, Two Tailed Test, Control, Migration, In Vivo, Fluorescence